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Aging in Garter Snakes Comparative Genomics Primate Reproductive Aging Aging in Wild Primates

Research in the Bronikowski lab incorporates evolutionary and ecological perspectives on life histories. We take an integrated approach to understanding phenotypic variation that varies with age, with recent emphases on senescence.  We address our life history questions at multiple levels of organization: evolutionary time scales, landscape ecological drivers, physiological  and cellular mechanisms, and genomic modifications in vertebrates. We answer fundamental questions in aging evolution and ecology using field studies of natural populations, physiological and molecular experimentation, and mathematical modeling. We focus currently on the late-life history, and are primarily interested in the evolution of senescence and lifespan. Our current projects involve (i) characterizing the action of aging genes and molecular networks in reptiles, (ii) testing how reptiles age mechanistically, considering oxidative stress and immune function, and (iii) modeling senescent rates in mortality and reproduction wild and semi-provisioned primates.

The integration of immune function, cellular stress response, and aging in wild populations of garter snake (Thamnophis elegans).

Field Site: Eagle Lake, CaliforniaLong-term ecological monitoring of multiple populations of garter snake in the Sierra Nevada mountains have revealed two life-history ecotypes located on the ends of the slow-to-fast pace of life. Fast-paced snakes have fast growth, early maturation, annual and high-effort reproduction, and short lifespan. Slow-pace of life snakes have delayed maturation, infrequent reproduction, and live twice as long as the fast-paced ecotype. These two life history ecotypes result from segregating alleles for growth.

We are asking how immune function (innate and acquired) and cellular stress response (DNA repair efficiency and oxidative stress potential) trade-off with reproduction and survival in these two ecotypes. At the same time, we are researching the endocrinological modulation of these trade-offs by examining the HPA (corticosterone) stress response and how insulin/insulin-like growth factor signaling regulates important life transitions in these organisms.

Summary of differences between slow-living and fast-living garter snake ecotypes around Eagle Lake, CA [pdf]

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Comparative Genomics of Aging genes in the reptiles.

We are broadly interested in understanding the molecular evolution and comparative genomics of stress and aging molecular networks across the reptile phylogeny and in comparison to mammals. Most major taxonomic groups have variable senescence rates and life spans. However, reptiles possess remarkable plasticity in their stress responses.  We believe this recommends reptiles as a model clade in which to understand the coevolution of stress response and aging pathways. We utilize genome sequencing, quantitative transcriptome analyses, and gene capture technology to characterize the genetic landscape of molecular networks in reptiles.


Bronikowski Data Server

Text Box: Preliminary results from sequencing the garter snake transcriptome, from normalized cDNA and 454 GS-FLX Titanium chemistries. Thus far, 60% of contigs have been assigned to known sequences in NCBI. Additional contig clustering and annotation is underway.  Number of Reads	1,237,007  Average Read Length	366  Total Mbp of Data	446  Number of Contigs	82,134  Total Mbp in Contigs	48  Contig Length (bp)	90-10,680 (avg 581)  Average Coverage	7.6    Table 1: Preliminary results from sequencing

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 Biodemographic indices of reproductive senescence in baboons

Semi-natural population of baboonsAs part of a large collaborative project, lead by Dr. Marc Tatar and the Southwest Foundation for Biomedical Research, we are developing the baboon as a model for studying human aging. Our work focuses on the biodemographic indices of reproductive senescence. Specifically, our reproductive aging project involves hormonal profiling of aging female baboons and their longitudinal decline in fecundability, ending ultimately in the menopause.

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Actuarial aging in wild primate populations.

In collaboration with many PIs that have long-term censuses of wild primates, we are modeling the rate of actuarial and reproductive senescence in representative species across the primate phylogeny.

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