DNA Extraction
Wendel Lab
Department of Ecology, Evolution and Organismal Biology
Iowa State University, Ames, IA 50011, USA

A couple notes on using Qiagen's Plant DNeasy extraction kit for DNA extractions... the protocol states that you can extract up to 100 milligram of fresh tissue using the mini kit (or 1 gram for the maxi kit). We have found that if the amount of starting tissue is reduced to 75% of the maximum (75mg for the mini kit or 0.75g for the maxi kit), the extraction works a little better. Also, in step 4 you have the option of spinning your sample before placing the supernatant onto the lilac shredder column. We always perform this optional step.