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RNA Gels With Formaldehyde Electrophoresis

Wendel Lab
Department of Ecology, Evolution and Organismal Biology 
Iowa State University, Ames, IA 50011, USA

Note: This protocol is based on protocols from the Wurtele Lab & Chapter 7, Molecular Cloning, Third Ed.

<!>All steps involving formaldehyde or formamide should be carried out inside a hood. 
<!> Formaldehyde causes Emphysema
<!> Do not use latex gloves with organic solvents - they will melt to your skin. Use nitrile. Read more about RNA Gels With Formaldehyde Electrophoresis

Total Protein Extraction: Phenol-Ammonium Acetate/Methanol Method

Wendel Lab
Department of Ecology, Evolution, and Organismal Biology
Iowa State University, Ames, IA 50011, USA

Materials and Reagents

1. Unless otherwise stated, all solutions were prepared in deionized water (18.2 M W conductivity). 

2. Filter sterilization solutions using either 25 mm syringe filter (0.2 µm, nylon; Fisher Scientific, Houston, TX, USA; product number, 09-719C) or steritop (0.22 µm; Millipore Corporation, Billerica, Massachusetts, USA; product number, SCGPS02RE) depending on solution volume.  Read more about Total Protein Extraction: Phenol-Ammonium Acetate/Methanol Method